| Penulis/Author |
MULYA FITRANDA AR (1); Prof. Dr. drh. Siti Isrina Oktavia Salasia (2) ; Prof. Dr. dr. Osman Sianipar, M.Sc., Sp.PK. (3); drh. Fatkhanuddin Aziz, M.Biotech., Ph.D (6); drh. Fajar Budi Lestari, M.Biotech. (8); Dr. drh. Christin Marganingsih Santosa, M.Si. (9) |
| Abstrak/Abstract |
Background and Aim: The emergence of methicillin Resistant Staphylococcus aureus (MRSA) as highly pathogenic strain in both veterinary and human medicine is currently a growing global problem. The aim of this study was to evaluate MRSA isolates of human and animal origin against various antibiotics in Yogyakarta, Indonesia.
Material and Methods: The susceptibility test was carried out by disk diffusion method using Mueller Hinton Agar (MHA) against 9 antibiotic discs. The MRSA strains were genetically confirmed through mecA gene detection encoding for methicillin resistance by Polymerase Chain Reaction (PCR).
Results: All 240 S. aureus strains isolated from animals were resistant to penicillin (100%) and 99% for human medical cases, followed by ampicillin, amoxycillin, oxacillin, erythromycin, clindamycin, tetracycline, gentamicin, ciprofloxacin in both human and animal isolates. A total of 83 MRSA were resistant to oxacillin, and penicillin G (100%), ampicillin (99.27%), amoxycillin (95.52%), erythromycin (87.77%), tetracycline (71.33%), clindamycin (63.24%), gentamycin (38.81%) and ciprofloxacin (26.87%).
Conclusion: Antimicrobial resistance pattern of S. aureus human isolates tends to be similar with their animal counterpart, with 77.20% of MRSA strains were classified as multi-drug resistance bacteria. These findings indicate an increase of multi-drug resistance S. aureus strains from animal origin in Yogyakarta, and thus, raises public health concern of MRSA zoonotic spread.
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