Maternal Low-Protein Diet Affects Folliculogenesis and Mitochondrial Distribution Within Ovarian Follicles in Adult Offspring of Mice
Penulis/Author
Rahmadsyah (1); dr. Junaedy Yunus, M.Sc., Ph.D. (2); MOHAMMAD GALIH P (3); dr. Agung Dewanto, Sp.OG(K)., Ph.D (4); Dr. dr. Shofwal Widad, Sp.OG(K). (5); Mochamad Anwar (6)
Tanggal/Date
2022
Kata Kunci/Keyword
Abstrak/Abstract
Introduction: Maternal nutrition intake affects the growth and development of foetal health. Poor nutritional intake
contributes to adverse metabolic outcomes in the offspring at an advanced age, including its reproductive function.
Maternal low-protein diet has been indicated to impair mitochondrial genetic expression in the offspring. This study
aims to evaluate the impact of maternal low-protein diet on the folliculogenesis and mitochondrial distribution within ovarian follicles in female adult offspring of mice. Methods: Swiss Webster mice were nourished either normal
protein diet containing 20?sein or low-protein diet (10?sein) during pregnancy and breastfeeding periods.
Female offspring were weaned onto the control diet until 8 weeks of age. Female adult offspring were injected with
20 IU of FSH intraperitoneally to induce folliculogenesis. Folliculogenesis was assessed by morphological observation under a light microscope using haematoxylin-eosin staining, while mitochondrial distribution within ovarian
follicles was identified by immunohistochemical staining of mitochondrial marker (ATP5A1). Immunostaining data
were presented as fraction area (%) and quantified using ImageJ software. Results: The results from the low-protein
diet group revealed a non-significant lower number of follicles at various stages compared to the normal protein
diet group (p>0.05). The immunopositive expression of the mitochondrial marker in the low-protein diet group was
notably lower than the control group (25.66% ± 4.90 vs 51.05% ± 1.51; p<0.05). Conclusion: Our study indicates
that maternal low-protein diet during pregnancy and breastfeeding impairs folliculogenesis and mitochondrial distribution within the ovarian follicles in mice offspring later in life.
Rumpun Ilmu
Ilmu Kedokteran Dasar & Biomedis
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Maternal Low-Protein Diet Affects Folliculogenesis and Mitochondrial Distribution Within Ovarian Follicles in Adult Offspring of Mice.pdf
