Manuka Honey Inhibits Biofilm Formation and Reduces the Expression of the Associated Genes in Pectobacterium brasiliense
Penulis/Author
Prof. Dr. Tri Joko, S.P., M.Sc. (1); SHEILA AVA (2); ISNA NURIFA SASMITA PUTRI (3); Prof. Dr. Ir. Siti Subandiyah, M.Agr.Sc. (4); Muhammad Saifur Rohman, S.P., M.Si., M.Eng, Ph.D. (5); Naoto Ogawa (6)
Tanggal/Date
1 2024
Kata Kunci/Keyword
Abstrak/Abstract
Biofilms are major virulence factors formed by pathogenic bacteria to invade their host and maintain their colony. While biofilms
usually develop on diverse solid surfaces, 2oating biofilms, also called pellicles, are formed at the air–liquid interface. To address
the problem of biofilm formation by bacterial pathogens, honey has been extensively studied. However, information on the e7ect
of honey on biofilm formation by plant pathogens is scarce. This study aimed to determine the effects of manuka honey on biofilm
and pellicle formation by Pectobacterium brasiliense and analyze the expression of genes encoding proteins needed to form biofilm
by using semiquantitative PCR and RT-qPCR. Treatment with 5% (w/v) of manuka honey significantly decreased biofilm and
pellicle formation by P. brasiliense. RT-qPCR results showed that the expression of bcsA, fis, hrpL, and expI decreased 7.07-fold,
5.71-fold, 13.11-fold, and 6.26-fold, respectively, after exposure to 5% (w/v) manuka honey. Our findings reveal that manuka
honey may effectively inhibit biofilm and pellicle formation.
Rumpun Ilmu
Ilmu Hama dan Penyakit Tanaman
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Scientifica - 2024 - Joko - Manuka Honey Inhibits Biofilm Formation and Reduces the Expression of the Associated Genes in.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
