Macrophage Immunomodulatory Activity of Unsaturated Fatty Acid Isolated from the Crown-of-thorns Star Fish (Acanthaster planci)
Penulis/Author
M. JANIB ACHMAD (1); Prof. Dr. Ir. Alim Isnansetyo, M.Sc. (2); Apt Noer Kasanah, S.Si., M.Si., Ph.D. (3); Prof. Dr. Ir. Ustadi, M.P. (4)
Tanggal/Date
2018
Kata Kunci/Keyword
Abstrak/Abstract
Introduction: Immunomodulator are chemical compounds that can improve the body’s defense
mechanisms both specific and non-specific, and non-specific induction of both cellular and
humoral defense mechanisms. Objectives: The objectives of this study were to investigate
immunomodulator activity and to identify the chemical constituents of active fractions from
star fish Acanthaster planci, based on bioassay guided isolation. Materials and Methods:
A. planci was collected from Ternate Island, North Moluccas, extracted with distilled methanol,
partitioned with gradient chloroform-hexane-water and fractionated in column chromatography
using silica gel and gradient hexane-ethyl acetate. Profiling chemical constituent was
done by thin layer chromatograpahy and GC-MS. The immunomodulator activity was measured
based on percentage of phagocytic capacity (PC) and phagocytic index (PI). Results: The
result showed that 3 partition fractions exhibited immunomodulator activity. Data analysis
exhibited that the best fraction was hexane fraction, and the best dosesmost effective doses
of PC and PI were at 0.5 mg/kg body weight (BW) and 0.7 mg/kg BW, respectively. Data analysis
of the 3 hexane fractions exhibited that the best fraction was fraction 3 and the best doses
of PC was at 0.5 mg/kg BW and that of PI was at 0.7 mg/kg BW. Metabolites analysis using
GC-MS yielded a number of chemical constituents of fraction 2 dan fraction 3 that dominated
by unsaturated fatty acid. The study concluded that star fish A. planci from Ternate Island has
a potential source of immunomodulator.
