| Abstrak/Abstract |
Lipase is an industrially important enzyme and usually obtained from microorganisms, especially filamentous fungi, which can produce it in a high amount. The method commonly used to produce lipase from filamentous fungi is Submerged Fermentation (SmF) with agitation. However, agitation require energy and increase the production cost. Hence, reducing the use of agitation is needed. This research aims to observe the influence of agitation speed toward colony growth, lipase productivity and nutrient absorption from Indonesian Aspergillus aculeatus Ms.11 in broth medium. A. aculeatus Ms.11 was inoculated into 50 mL fermentation medium which contains 1% of olive oil and glucose mixture with comparison 1:1. Incubation was done using varied agitation speed (0, 50, 100, 150 and 200 rpm) at 30?C for 96 hours. Relative activity lipase productivity, enzyme-specific activity, and glucose and nitrogen consumption were determined after the incubation. The results show that the highest lipase activity (6.33 U/mL) and lipase specific activity (0.10 U/mg) were observed on 0 rpm, while the highest lipase productivity (0.43 U/mg) was observed on 50 rpm. We concluded that maximum lipase production from Ms. 11 can be obtained using low agitation speed. Further study to optimize carbon and nitrogen sources is suggested. |
