| Judul/Title |
Kinetic studies on the transesterification of sunflower oil with 1-butanol catalyzed by Rhizomucor miehei lipase in a biphasic aqueous-organic system |
| Penulis/Author |
Dr. Miftahul Ilmi, S.Si., M.Si. (1); A. Hommes (2); H.J. Heeres (4); Prof. Dr. Ir. Chusnul Hidayat (5); A. Hommes (6) |
| Tanggal/Date |
2016 |
| Kata Kunci/Keyword |
|
| Abstrak/Abstract |
The kinetics of sunflower oil transesterification with 1-butanol using a homogeneous lipase (Rhizomucor miehei) in an aqueous-organic biphasic system were studied in a stirred batch reactor set-up. An initial screening study was performed to optimize relevant process conditions (enzyme concentration, stir- ring speed, 1-butanol to oil ratio). A kinetic model was developed based on concentration time profiles. Important reaction parameters were the oil concentration in n-hexane, the enzyme concentration, the 1-butanol to oil molar ratio; the organic to an aqueous solvent volume ratio, the temperature, the pH setting using a phosphate buffer and the agitation rate. The experimental data were modelled using the Ping Pong Bi Bi mechanism with non-competitive inhibition by 1-butanol and a term for irreversible enzyme deactivation during reaction. Agreement between model and experiments was good (R2 = 0.99, average error 4.2%).
© |
| Rumpun Ilmu |
Biologi (dan Bioteknologi Umum) |
| Bahasa Asli/Original Language |
English |
| Level |
Internasional |
| Status |
|
