| Abstrak/Abstract |
Fruit consumption such as strawberries always increase in Indonesia and reaching about 210
tons in 2011. Strawberry (Fragaria vesca L. subsp. californica Cham. and Schltdl. cv. Californica)
has been developed in Indonesia, especially in Banyuroto, Sawangan, the district of Magelang,
Central Java. In order to improve the fruit productivity (quality and quantity), genetic
characterization including chromosomes character of this fruit are needed to be investigated.
Therefore, the research was conducted to determine the chromosomes number, chromosomes size,
chromosomes shape and karyotype of control and strawberries treated by colchicine as mutagenic
agent. The method was squash and flow cytometry method. Whereas the cell suspension sample
were observed used flow cytometer instrument. The result of research shown that the chromosome
number of strawberry Californica in normal and treatment strawberries was 2n=14. The
chromosomes shape of normal strawberry was 10 metacentric and 4 submetacentric. Whereas the
chromosomes shape of treatment strawberries used colchicine was 10 metacentric and 4
submetacentric. Flow cytometric analysis shown that control strawberries and treated strawberries
has diploid ploidy level.
Keywords: chromosomes, colchicine, flow cytometry, polyploidization, strawberry Californica |
