Jatropha curcas leaves exert anti-arthritic activity on adjuvant-induced arthritis in rats
Penulis/Author
HANIF NASIATUL BAROROH (1); Iskandar Sobri (2); EKA PRASASTI NUR RACHMANI, S.SI.,APT. (3); Prof. Dr.rer.nat. apt. Triana Hertiani, S.Si., M.Si. (4); Prof. Dr. apt. Zullies Ikawati. (5)
Tanggal/Date
2014
Kata Kunci/Keyword
Abstrak/Abstract
BACKGROUND
Jatropha curcas leaves have been proven to be anti-inflammatory and
antioxidant. In this study we examined the antiarthritic effects of ethanolic
extract of J. curcas leaves using adjuvant induced arthritis (AIA) in rats.
METHODS
Male Wistar rats were divided into 6 groups (n=8), consisting of normal group
(0.9% NaCl), control group (complete Freund’s adjuvant/CFA 1 mg/ml), sodium
diclofenac group at a dose of 6.75 mg/kg (p.o), ethanolic extract of J.curcas
groups at doses of 150 mg/kg (p.o), 300 mg/kg (p.o) and 600 mg/kg (p.o).
Each group was induced by 0.2 ml CFA on day 1 and a booster injection on day
5. Extracts of J. curcas were administered on days 14-28. Arthritic scores were
determined, then analyzed using Kruskal Wallis followed by Mann Whitney
tests. Mobility scores were analyzed using one way analysis of variance, followed
by least significant difference multiple comparison test. Arthritic joint
histopathology was observed on day 29.
RESULTS
The results showed that the ethanolic extract of J. curcas leaves at doses of
150 mg/kg, 300 mg/kg and 600 mg/kg significantly reduced arthritis scores
(p<0.05) compared to control group (CFA). The J. curcas leaf extract at doses
of 150 and 300 mg/kg BW decreased mobility scores. Histopathology studies
showed that the J. curcas extract reduced edema and cartilage destruction in
arthritic joints.
CONCLUSIONS
The J. curcas leaf extract had anti-arthritic effects by reducing arthritis scores
and mobility scores. The extract should be further examined as a potential
candidate for anti-arthritic therapies.
