| Abstrak/Abstract |
Actinomycetes are filamentous Gram-positive bacteria that potential sources of
antimicrobial substances. The aim of this study was to isolate actinomycetes from different soil types
in Enggano Island and screened their antimicrobial activity. The phenol method with 802 medium
and humic acid vitamin (HVA) medium was used to isolate the actinomycetes. Furthermore,
molecular identifications were performed based on 16s rRNA gene sequence. Antimicrobial activity
screening was performed against Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.612,
Micrococcus luteus BTCC B.552, Staphylococcus aureus BTCC B.611, and Candida albicans BTCC
Y.33 by using the agar plug diffusion method. Fifty-six actinomycetes isolates were obtained from
11 soil types. Most of the isolates were Streptomyces genus with species S. hydrogenans, S.
coelicolor, and S. koyangensis, which have similarities in the 16S rRNA gene sequence ranging from
98 -100%. Among isolates screened for their antimicrobial activity, five isolates, i.e. SMP 6, SMP
7b, SMP 18, SHP 9-3, and SHP 22-1a showed a potential activity against test microorganisms
selected. All of isolates were more active against C. albicans compared with the tested bacteria.
Further study will be conducted to isolate active substances against C. albicans. |
