Isolation, and Selection of Proteolytic Lactic Acid Bacteria from Colostrum of Dairy Cattle
Penulis/Author
ANGGA RIZKI RAMADHAN (1); Prof. Dr. Ir. Zaenal Bachruddin, M.Sc., IPU., ASEAN Eng. (2); Prof. Widodo, S.P., M.Sc. Ph.D. (3); Prof. Ir. Yuny Erwanto, S.Pt., M.P., Ph.D., IPM (4); Dr. Ir. Chusnul Hanim, M.Si., IPM., ASEAN Eng. (5)
Tanggal/Date
3 2020
Kata Kunci/Keyword
Abstrak/Abstract
Lactic Acid Bacteria (LAB) are bacteria that produces lactic acid and are very useful bacteria, one of which can act as a probiotic because it is able to produce bacteriocins and produce peptide compounds with high biological activity. Colostrum is the first fluid that comes out of the udder of the mother cattle after giving birth which is very good for newborns because it contains antibodies. This research aims to isolation, selection and identify the types of LAB that found in dairy cow colostrum, especially LAB which have superior proteolytic activity. LAB obtained is expected to have a positive effect on livestock productivity. This research was conducted In-vitro in a laboratory. The research procedure included the isolation of LAB from the colostrum of dairy cows, then the selection procedure by looking at the best protease enzyme activity qualitatively and quantitatively, and testing for resistance to low pH. Lactic acid isolation, selection and identification were analysed using descriptive analysis and quantitative analysis using one-way ANOVA for Completely Randomized Design (CRD). The results obtained 6 superior proteolytic LAB isolates based on the protease enzyme activity coded BAL Pro 4, BAL Pro 3, BAL Pro 8, BAL Pro 9, BAL Pro 21 and BAL Pro 20.
