| Abstrak/Abstract |
Alzheimer's disease (AD), which is characterized by abnormal amyloid (A) peptide deposition and intracellular accumulation of neurofibrillary tangles (NFT) of hyperphosphorylated tau protein, is the most prevalent cause of dementia in people older than 65 years old globally. According to Alzheimer's Disease International, there are currently more than 55 million people living with dementia worldwide. By 2050, it is predicted that this number will rise to 152 million. Although synthetic medications are readily available, the majority of these are recommended to treat symptoms, with infertility being the most common side effect. Due to their comparatively low side effects, the need for searching for natural treatments derived from plant sources is therefore continuing to grow. Different plant species that have the greatest therapeutic potential against neurodegenerative diseases have been researched. Among them, white cabbage (Brassica oleracea var. capitata f. alba), red onion (Allium caepa) and coffee (Coffea robusta) are rich sources of sinigrin, quercetin, and chlorogenic acid, respectively, and can be considered as a combination therapy to treat cognitive dysfunction. Based on their previous interaction with phosphodiesterase-4 (PDE4) interaction in vitro, the present study is aimed at the identification and quantification of sinigrin, quercetin and chlorogenic acid in white cabbage, red onion and coffee extracts, respectively, by liquid chromatography high resolution mass spectrometry (LC-HRMS), and in vivo determination of cognitive enhancing activity of these three extracts in mice model via cAMP/PKA/CREB/BDNF Pathway. Seven weeks to two months old, 50 male Balb/c mice will be employed in this study. The mice will be divided into 5 distinct groups, each with 10 mice. Scopolamine HCl will be used to induce amnesia and rolipram as standard drug to treat cognitive dysfunction in mice. The study will be carried out for 14 days. Furthermore, targeting cellular molecules such as cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), and phosphorylated cAMP response element binding (pCREB) and brain derived neurotrophic factor (BDNF) levels will be assessed in hippocampal tissues by using different standard testing kits to determine the cellular molecular mechanism after sacrificing mice. As per previous reports, there has not any such study been performed yet. Therefore, study on BCA leaves extract is necessary. |
