| Abstrak/Abstract |
Kyuri green mottle mosaic virus (KGMMV) is one of the virus which can reduce the quality and the amount of melon production in Indonesia. Resistance breeding is one of the solution to overcome the disease by crossing of PI 371795 and Andes to study the inheritance of resistance gene to KGMMV in melon. The aims of this research were to study the inheritance of resistance gene to KGMMV and to study various phenotypes of melon fruit produced by crossing between PI 371795 and Andes. Reciprocal breeding experiments were conducted between PI 371795 and Andes to obtain F1 population. Furthermore, F1 individuals were selfed to produce F2 population. On the other hand, another F1 individual was crossed to Andes to produce test cross population. F1, F2 and test cross individuals were then inoculated manually by KGMMV melon isolate (KGMM-YM). Resistance to KGMMV-YM was analyzed by symptom observation and serological detection using double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). The DAS-ELISA results of F2 and test cross population were analyzed by chi-square test. Results of this research indicate that melon PI 371795 and F1 are resistance to KGMMV-YM, while Andes is susceptible. F2 and test cross population showed segregation and it follows Mendelian law with ratio F2 segregation is 3:1 (34 resistant, 17 susceptible) while the ratio in test cross is 1:1 (6 resistant, 7 susceptible). The results of this study revealed that the inheritance of resistance gene to KGMMV-YM is controlled by a single dominant gene and it is proposed to be called Krey for the allele symbol (KGMMV-YM resistance gene). Results of these breeding experiments also show four variations of melon fruits which have the potential to become a commercial melons. |
