| Abstrak/Abstract |
Original Article | JOGCR. 2025; 10(7): 532-536
Volume 10, July 2025 Journal of Obstetrics, Gynecology and Cancer Research
Journal of Obstetrics, Gynecology and Cancer Research | ISSN: 2476-5848
Induction of Fresh Chocolate Cyst Pulp into Endometriosis Mice Model
Ivanna Beru Brahmana1* , Soetrisno Soetrisno2, Mulyoto Pangestu3, Rita Cempaka4, Ika Puspitasari5
1. Department of Obstetrics and Gynecology, Medical Study Program, Faculty of Medicine and Health Sciences,
Universitas Muhammadiyah Yogyakarta, Indonesia
2. Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
3. Education Program in Reproduction and Development, Department Obstetrics and Gynecology, Monash University,
Australia
4. Department of Pathology Anatomy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
5. Faculty of Pharmacy, Academic Hospital, Universitas Gadjah Mada, Yogyakarta, Indonesia
Article Info ABSTRACT
10.24200/jogcr.10.7.532 Background & Objective: A chocolate cyst is a type of endometriosis in addition to
the appearance of endometrial lesions. The limitations of obtaining these samples led to
trying to induce fresh chocolate cyst pulp to become a model of endometriosis mice.
Chocolate cyst pulpy can be obtained through cyst puncture with no need to perform
laparoscopy or laparotomy. This study was conducted with the aim of proving that
induced mice with fresh chocolate cyst pulp samples succeeded in becoming
endometriosis models of mice.
Materials & Methods: Fresh chocolate cyst slurry was injected as much as 5.10-3 mL/g
of mice weight intraperitoneally on day zero. Mice of Mus musculus strain Balb/c were
injected with cyclosporine 1.4.10-2 mg/g of the weight of mice intraperitoneally daily
for 14 days. On the 1st and 5th day, the mice were injected with estradiol 2.6.10-4 mL/g
of the weight of the mice, intramuscularly. Induction research on the endometriosis
mice model was conducted at the Pharmacology Laboratory of the Universitas Gadjah
Mada, Yogyakarta, Indonesia. On the 15th day, the mice were necropsied for
histopathological examination at the Anatomical Pathology Laboratory, Faculty of
Medicine, Public Health & Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia,
with Hematoxylin-Eosin staining.
Results: A total of 6 mice induced with fresh chocolate cyst pulp histopathologically
showed signs of endometriosis. Histopathologically readings showed external
endometriosis cyst (1 mouse), endometriosis tissue (1 mouse), hemosiderosis (1
mouse), and stromatosis (3 mice).
Conclusion: The six mice were induced with fresh chocolate cyst slurry were 100%
successful in becoming endometriosis mice models, as a novelty in endometriosis
studies using experimental animals |
