In-vitrocytotoxicity and apoptosis mechanism of ethyl acetate extract from Trichoderma reeseistrain TV221 associated with marine sponge: Stylissa flabelliformis
Penulis/Author
Prof. Dr. apt. Erna Prawita Setyowati, M.Si. (1); Dr. Sylvia Utami Tunjung Pratiwi, S.Si., M.Si. (2); Dr. Purwantiningsih, S.Si., M.Si., Apt. (3); Dr. apt. Indah Purwantini, S.Si., M.Si. (4)
Tanggal/Date
2018
Kata Kunci/Keyword
Abstrak/Abstract
This research aimed to acknowledge the cytotoxic effect of ethyl acetate extract fermented from Trichoderma reesei
strain TV221 fungi associated with Stylissa flabelliformissponge in various cell lines such as WiDr, T41, T47D, Raji,
and Vero, as well as to discover the mechanism of cell’s death. The result of fermented fungi T. reeseiwas extracted
with ethyl acetate. Ethyl acetate extract was tested against cell lines using the MTT assay. The result of cytotoxic
selectivity test with lowest IC50 price, then apoptosis test with double staining acridine orange-ethidium bromide
method was performed Ethyl acetate extract fermented from T. reeseihas cytotoxic ability against tumor cell lines
(WiDr, T41, T47D, Raji) and is not toxic to normal cell lines (Vero). The test sample can spur apoptosis process on
colon cancer cells Widr
Rumpun Ilmu
Biologi Farmasi
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
In-vitro cytotoxicity and apoptosis mechanism of ethyl acetate extract from Trichoderma reesei strain TV221 associated with marine sponge_ Stylissa flabelliformis.pdf
