In Vitro Immunomodulatory and Cytotoxic Potentials of Faloak (Sterculia quadrifida R.Br.) Bark
Penulis/Author
Prof. Dr.rer.nat. apt. Triana Hertiani, S.Si., M.Si. (1); Dr. Purwantiningsih, S.Si., M.Si., Apt. (2); AJI WINANTA (3); WIDYANDANI SASIKIRANA (4); RIMA MUNAWAROH (5); Prof. Dr. apt. Erna Prawita Setyowati, M.Si. (6); drh. Retno Murwanti, M.P., Ph.D. (7); SISWADI (8)
Tanggal/Date
5 2019
Kata Kunci/Keyword
Abstrak/Abstract
Faloak (Sterculia quadrifida R.Br., Sterculiaceae) is an
Indonesia indigenous plant widely used as a traditional remedy for various
health complaints. This study aimed to explore the immunomodulatory and
cytotoxicity potentials of Faloak bark by using in vitro techniques. Barks
was extracted using water, ethanol and ethanol: water (1:1) separately.
Colorimetric analysis was used for the determination of the total phenolic
and the total flavonoid contents. Immunomodulator activities of the extracts
were evaluated by studying their effects on mice macrophages and
lymphocytes using in vitro methods, while the cytotoxicity potential was
evaluated against Vero and HepG2 cell lines. The active extract was further
fractionated and tested using different cell lines by the MTT method. Data
were analyzed using one-way ANOVA and LSD tests, with a 95%
confidence level (p<0.05). All extracts significantly increased the
phagocytosis of macrophages compared to that of control in a concentrationdependent manner. However, all the extracts exhibited the Stimulation Index
value of < 2, which indicated no effect on lymphocyte proliferation. The ethyl
acetate fraction showed the highest activity against T47D, MCF7 and HepG2
cells with IC50 9.56; 7.62; 3.24 µg/mL and the Selectivity Index (against
Vero cells) as follows, 2.01; 2.52; 5.94, respectively. Faloak bark extracts can
stimulate macrophage phagocytosis activity in vitro, which positively
correlates to the contents of flavonoids and other phenolic compounds. The
ethyl acetate fraction exhibited the highest activity, which supports its
potency as cancer chemopreventive herbal medicine.
Rumpun Ilmu
Biologi Farmasi
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
OJBS2019.pdf
Bukti Accepted
2
In Vitro Immunomodulatory and Cytotoxic Potentials of Faloak (Sterculia quadrifida R_Br_) Bark.pdf
[PAK] Cek Similarity
3
Bukti Korespondensi OJBS_full.pdf
[PAK] Bukti Korespondensi Penulis
4
EC-Aji Winata.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
5
ilovepdf_merged (8).pdf
[PAK] Full Dokumen
6
In vitro immunomodulatory and cytotoxic potentials of faloak.pdf
[PAK] Full Dokumen
7
In Vitro Immunomodulatory and Cytotoxic Potentials of Faloak (Sterculia quadrifida R_Br_) Bark.pdf
