Karya
Judul/Title In vitro antiplasmodial activity and cytotoxicity of active subfractions of harmsiopanax aculeatus leaves
Penulis/Author RACHEL TURALELEY (1); Dr. dr. Mahardika Agus Wijayanti DTM&H., M.Kes. (2); Prof. Dr.rer.nat. apt. Triana Hertiani, S.Si., M.Si. (3); Prof. Dr. Mustofa, Apt., M.Kes. (4)
Tanggal/Date 17 2020
Kata Kunci/Keyword
Abstrak/Abstract armsiopanax aculeatusleaves, a medicinal plant with locally named kapur, have been used traditionally to treat malaria in Maluku, Indonesia. However, the scientific information of this plant is still limited. In our previous study, the methanol extract of this plant leaveshave been proven to possess in vitroantiplasmodial activity. This study was conducted to evaluate in vitroantiplasmodial activity and cytotoxicity of subfractions of the plant leaves. Fractionation was performed using a column chromatography with Sephadex LH-20 as the stationary phase and methanol as the mobile phase. The subfractionsobtained were then tested for in vitroantiplasmodial activity on a chloroquine-resistant FCR3 strain of Plasmodium falciparumusing a visual method. Cytotoxicity was evaluated by using MTT assay. The in vitroantiplasmodial activity and cytotoxicity wereexpressed as IC50, calculated using probit analysis with SPSS 16 for windows. The results showed that the four subfractions tested have a high antiplasmodial activity with IC50values of 0.09; 0.18; 0.01; and 0.77 ?g.mL-1, respectively. In addition, thesesubfractions had IC50values of >400 ?g.mL-1against Vero cells indicating that they were non-toxic. In conclusion, the subfractions of H. aculeatusleaves are very active and selective against P. falciparum. Further study will be conducted to isolate theactive compounds.
Rumpun Ilmu Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
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