| Penulis/Author |
NOOR ARINTA SARI M (1); Dr.rer.nat. Ronny Martien, M.Si. (2); Prof. Dr. Ir. Zuprizal, DEA., IPU., ASEAN Eng. (3); Ir. Nanung Danar Dono, S.Pt., M.P., Ph.D., IPM., ASEAN Eng. (4) |
| Abstrak/Abstract |
Purpose of this study was to examine the effect of nanoencapsulation technology in
protecting the bioactive components of mindi (Melia azedarach Linn.) leaf extract. The first
step was formulation of the ratio of mindi leaf extract:chitosan:sodium tripolyphosphate
(STPP) for nanoencapsulation by ionic cross-linking. The results showed that the optimum
formulation ratio of mindi leaf extracts:chitosan:STPP was 1:7:1/175. Nano-capsulation
characteristics of the fitobiotic was measured (particle size (PSA), zeta potential value,
morphology of nanoencapsulation (TEM), and bacterial growth inhibition). The second step
was measuring the bacterial growth inhibition using the well diffusion technique with six
treatments and three replications. The treatments were: aquadest (T1), aquadest with 100 ppm
antibiotic Tetracycline addition (T2), aquadest with 0,2% chitosan (T3), aquadest with 0,04%
STPP (T4), aquadest with 2% mindi leaf extract (T5); and aquadest with nanoencapsulated
mindi leaf extract (T6). The results showed that particle size of nanoencapsulation mindi leaf
extract was 535.2±12.83 nm with Polydispersity Index (PI) 0.436±0,21 and zeta potential value
59.73±0.35 mV. The formulation of mindi leaf extract was found to possess inhibitory activity
against Escherichia coli, Lactobacillus acidophilus, and Salmonella typhimurium. The research
concluded that formulation of mindi leaf extract was clear, stabile and had antibacterial
activity. |
