In vitro antibacterial activity of methanol extract of a sponge, Geodia sp. against oxtetracycline-resistant Vibrio harveyi and its toxicity
Penulis/Author
Prof. Dr. Ir. Alim Isnansetyo, M.Sc. (1); Drs. Trijoko, M.Si. (2); Prof. Dr. apt. Erna Prawita Setyowati, M.Si. (3)
Tanggal/Date
2009
Kata Kunci/Keyword
Abstrak/Abstract
Abstract: In this study, the extract was tested for in vitro activity against oxytetracycline-resistant V. harveyi. Toxicity of the methanolic extract was evaluated by Brain Shrimp Lethality test. Geodia sp. was characterized by three spicula types (oxeas, trianes and oxyaster euaster), encrusting growth formation, hispid surface features and skeletal structure of paratangential ectosome. The methanolic extract of Geodia sp. exhibited anti-oxytetracycline-resistant V. harveyi activity with MIC of 31.25 ?g mL-1. The extract also was able to inhibit the growth of oxytetracycline-resistant V. harveyi in broth culture at concentrations of 1 and 2xMICs and able to kill almost V. harveyi cells at 4xMIC. Interestingly, the extract did not show any toxic effect in Artemia salina up to 125 ?g mL-1. It is the first report for the antibacterial activity of methanolic extract of Geodia sp. against oxytetracycline-resistant V. harveyi, a pathogenic bacterium in marine aquaculture. This results suggest that Geodia sp. might be used as a source of alternative compound to control marine bacterial pathogen especially oxytetracycline-resistant V. harveyi.
Rumpun Ilmu
Budidaya Perikanan
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
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COMPILED Article No_ 9087-JBS-ANSI.pdf
[PAK] Bukti Korespondensi Penulis
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1 Cover JBS.pdf
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3_ Editor in Board.pdf
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2 Daftar Isi.pdf
[PAK] Daftar Isi
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2009 Published paper JBS.pdf
Bukti Published
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Scimago J Industrial Microbil Biotechnol.pdf
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In vitro antibacterial activity of methanol extract of a sponge, Geodia sp_ against oxtetracycline-resistant Vibrio harveyi and its toxicity.pdf
[PAK] Full Dokumen
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In vitro antibacterial activity of methanol extract of a sponge, Geodia sp_ against oxtetracycline-resistant Vibrio harveyi and its toxicity.pdf