Karya
Judul/Title In Vitro and In Silico Studies of Secang Wood (Caesalpinia sappan L.) Extracts and Brazilin as Natural Phosphodiesterase-1 (PDE1) Inhibitor for Herbal Cognitive Enhancer Development
Penulis/Author HELMI (1); Prof. Dr.rer.nat. Nanang Fakhrudin, M.Si., Apt. (2); Prof. Dr. apt. Arief Nurrochmad, M.Si., M.Sc. (3); Dr. B.S. Ari Sudarmanto, M.Si. (4); Prof. Dr. apt. Zullies Ikawati. (5)
Tanggal/Date 20 2020
Kata Kunci/Keyword
Abstrak/Abstract Secang (Caesalpinia sappan L.) (CS) is one of the plants widely found in Indonesia and known to have a various biological activities. However, there is no scientific evidence regarding its cognitive enhancing activity through the inhibition of Phosphodisesterase-1 (PDE1). PDE1 is recently discovered as a promising molecular target for the development of cognitive enhancer agents to combat cognitive diseases. This study aimed to identify in vitro PDE1-inhibition activity of n-hexane, chloroform and ethanol extracts of CS wood and its major constituent, brazilin, with Cyclic Nucleotide Phosphodiesterase Assay Kit and in silico interaction of brazilin with PDE1 used Molecular Operating Environment (MOE) ver. 2016.01. This study demonstrates that the ethanol extract was the most active PDE1-inhibition activity compared to the other extracts with the inhibition of 52.7% at concentration of 100µg/mL. Interestingly, brazilin at concentration of 11.45µg/mL inhibited PDE1 activity (30.6%) equal to the positive control, vinpocetine (14.02 µg/mL). In conclusion, the ethanol extract of CS wood and its major constituent, brazilin, inhibited PDE1 activity and could be develop as cognitive enhancer.
Rumpun Ilmu Biologi Farmasi
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1helmi.pdf[PAK] Full Dokumen
227_In Vitro and In Silico Studies of Secang Wood (Caesalpinia sappan L.pdf[PAK] Cek Similarity
327_editorial.pdf[PAK] Halaman Editorial
427_ sampul.pdf[PAK] Halaman Cover
527 daftar isi.pdf[PAK] Daftar Isi
627a_In Vitro and In Silico Studies of Secang Wood (Caesalpinia sappan L.pdf[PAK] Cek Similarity