Abstrak/Abstract |
This research aimed to determine the adhesion of Bacillus sp. (PCP1) and
Enterobacter sp. (JC10) on intestinal epithelial cells of red tilapia (Oreochromis sp.) and the
effect of the probiotics application in feed on fish growth, survival rate, and feed conversion
ratio. In vitro adhesion test was performed by using 108
cells/ml of bacteria, and 105
cells/ml of
epithelial cells for 1 hour of incubation. The probiotics were added to the fish pellet with the
dose of 5 x 104
CFU/g of feed with four treatments, including probiotic application every three
days, seven days, without probiotic, and commercial probiotic application every three days. Each
treatment consists of three replications. Red tilapia is maintained for 30 days in fiberglass ponds.
The feed is given two times per day with a dose of 5 % of the biomass. The adhesion experiment
results showed that Bacillus sp. (PCP1) and Enterobacter sp. (JC10) have adherence abilities
higher than the commercial probiotics. The application of probiotics in tilapia for one month did
not affect the fish growth, survival rate, and feed conversion ratio (P > 0.05). Probiotic
application in a longer period is needed to be addressed. |