| Abstrak/Abstract |
Inhibition of Angiotensin Converting Enzyme (ACE) plays an important
role to treat hypertension. Flavonoid compounds have known have ACE
inhibitor activity. The purpose of the present study was to evaluated
ACE inhibi tior activity of flavonoid-phenolic fraction from extract of 3
edible plants using in vitro assay.
The investigated samples were Vigna unguiculata, Allium fistulosum and
Manihot uti lissima. Flavonoid-phenolic compounds in samples were
analyzed using Thin Layer Chromatography and Densitometer. Total
phenolic contents were determined by Folin-Ciocalteu colorimetric
method. ACE inhibitory assay method is evaluated based on hydrolysis
of substrate HHL (Hippuryl-L-Histidyl-L-Leucine) by ACE, and
measuring the amount of HA (Hippuric Acid) product using
spectrophotometer at 228 nm. Then, ACE inhibitor activity was
determined by % ACE Inhibitor value.
The resul t of the present study showed that each fraction from Vigna
unguiculata, Allium fistulosum and Manihot utilissima extracts were
containing flavonoid-phenolic compounds wi th total phenolic contents
(per 100 g fresh weight) were 21,99±0,64 mg GAE, 66,29±1,79 mg GAE
and 113,12±1,29 mg GAE, respectively. ACE Inhibitor activity of
flavonoid-phenolic fraction from Vigna unguiculata, Allium fistulosum
and Manihot utilissima extracts at 100 ?g/ml concentration were
23,77±9,19 %, 86,75±12,44 % and 105,59±10,02 %, respectively. From
this study, flavonoid-phenolic fraction from Allium fistulosum and
Manihot utilissima extracts are potential to develop as antihypertensive
agent.
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