| Abstrak/Abstract |
Inhibition of Angiotensin Converting Enzyme (ACE) plays an important role to treat hypertension. Flavonoid compounds have been known to have ACE inhibitor activity. The purpose of the present study was to evaluate ACE inhibitior activity of flavonoid-phenolic fraction from extracts of three edible plants using in vitro assay. The investigated samples were Vigna unguiculata, Allium fistulosum and Manihot utilissima. Flavonoid-phenolic compounds in the extracts obtained from the samples were analyzed using Thin Layer Chromatography and Densitometer techniques. Total phenolic contents were determined by Folin-Ciocalteu colorimetric method. ACE inhibitory assay method was based on hydrolysis of substrate HHL (Hippuryl-L-Histidyl-L-Leucine) by ACE into HA (Hippuric Acid) product. The amount of HA (Hippuric Acid) represented ACE activity and it was measured using spectrophotometer at 228 nm. Then, ACE inhibitor activity was determined by ?E inhibitor value. The result of the present study showed that each fraction from Vigna unguiculata, Allium fistulosum and Manihot utilissima extracts contained flavonoid-phenolic compounds with total phenolic contents (per 100 g fresh weight) were 21.99± 0.64 mg GAE, 66.29±1.79 mg GAE and 113.12±1.29 mg GAE, respectively. ACE Inhibitor activity of flavonoid-phenolic fraction from Vigna unguiculata, Allium fistulosum and Manihot utilissima extracts at 100 ?g/mL concentration were 23.77±9.19%, 86.75±12.44% and 105.59±12.26%, respectively. From this study, flavonoid-phenolic fractions from Allium fistulosum and Manihot utilissima extracts might be potential to develop as antihypertensive agent. © 2016, International Journal of Pharmaceutical and Clinical Research. |
