Karya
Judul/Title IMMUNOSTIMULATORY EFFECT OF FATTY ACID FROM STARFISH (Acanthaster planci) ON LYMPHOCYTE PROLIFERATION IN-VITRO
Penulis/Author M. JANIB ACHMAD (1) ; Prof. Dr. Ir. Alim Isnansetyo, M.Sc. (2); Apt Noer Kasanah, S.Si., M.Si., Ph.D. (3); Prof. Dr. Ir. Ustadi, M.P. (4); Prof. Dr. Ir. Kamiso Handoyo Nitimulyo, M.Sc. (5)
Tanggal/Date 2014
Kata Kunci/Keyword
Abstrak/Abstract The aims of this study were to investigate lymphocyte proliferation activity and to identify chemical constituents of active fractions of starfish Acanthaster planci. Acanthaster planci was collected from Ternate Island, North Moluccas, extracted with distilled methanol and water, partitioned with gradient chloroform-hexane-methanol-water and fractionated in column chromatography using silica gel and hexane-ethyl acetate-methanol. The active compound had been purified by Thin Layer Chromatography (TLC) and identified by Gas Chromatography-Mass Spectrometry (GC-MS). The lymphocyte proliferation activity was measured based on % Stimulation Index (SI) from sample absorbency and control absorbency. The result showed that the 3 fractions of hexane fraction exhibited lymphocyte proliferation activity. Fraction 1 was able to increase lymphocyte proliferation at 48 hours and 72 hours by 55% (80 ?l/ml) and 88% (160 ?l/ml) increase, and fraction 2 had 77% (160 ?l/ml) and 86% (640 ?l/ml) increase. Meanwhile, fraction 3 had 75% (640 ?l/ml) and 89% (640 ?l/ml) increase. Metabolite analysis of active fraction using GC-MS yielded a number of chemical constituents that was dominated by fatty acid. The study concluded that starfish A. planci from Ternate Island has a potential source of immunostimulator.
Rumpun Ilmu Bioteknologi Perikanan
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
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