| Abstrak/Abstract |
Suji is a plant that has long been used as food colorant and medicinal plant in Indonesia. This study aimed to determine the immunomodulatory activity of fractions obtained from suji leaves by using in vitro phagocytic macrophage assay and also to determine the phytochemical compounds of the most active fraction. Initially, Suji dry leaves powder were macerated with 96% ethanol upon filtration, then the filtrate obtained was evaporated to dryness. The extract obtained was triturated with chloroform to give fraction soluble (F1) and insoluble fraction (F2). Then, F1 was partitioned using a mixture of hexane-methanol-water (25: 14: 1 v /v) to give two layers, namely upper layer (F3) and lower layer (F4). Furthermore, the four fractions (F1, F2, F3 and F4) were tested by in vitro phagocytic macrophages method at the concentration of 10, 25, 50 and 100 ?g/mL. The parameters used were the Phagocytic Index (PI) and the Phagocytic Capacity (PC). Macrophage phagocytic in vitro assay showed that fractions of the suji leaf ethanolic extract could improve the phagocytic activity of macrophages. The fraction that demonstrated the highest activity was subjected to phytochemical study by Thin Layer Chromatography (TLC). Based on the data obtained, F3 was the most active fraction, because it has the highest PC and PI values compared to other fractions or negative control. TLC test revealed that F3 contained terpenoids and flavonoids. |
