| Abstrak/Abstract |
Obesity is one of major nutritional problems in the world. Obesity
is very dangerous, especially when concentrated in the abdomen, because
it is closely linked to various diseases such as diabetes, hypertension,
heart disease, which can to causing death. This study aims to identify the
nature and distribution of ghrelin on gastric endocrine cells in the obese rat
(Rattus norvegicus) by using immunohistochemical techniques. The results
will strengthen the understanding of the role and function of ghrelin as an
alternative therapeutic target on obesity. The research used gastric tissues of
ten obese and control rats which were stained with avidin-biotin-peroxidase
complex method of immunohistochemistry. The results showed the existence
of two types of ghrelin-producing cells (open and closed types) on the gastric
mucosa of control rats, and only one type of ghrelin producing cells (open
type) in obese rats. The intensity of ghrelin immunoreactive positive cells was
detected weak in obese rats, but very strong in control rats. Ghrelin endocrine
cells mainly distributed in the basal part of the gastric mucosa of the fundus
parts, with a very small number in obese rats, but highly abundant in control
rats. This study confirmed the decrease of the ghrelin synthesis and secretion
in obese rat (Rattus norvegicus) at the cellular level. The decrease of ghrelin
synthesis is characterized by a reduction on the number of ghrelin producing
cells, the disappearance of the close type of ghrelin producing cells, and
the low activity of protein synthesis in the ghrelin producing cells. Ghrelin
endocrine cells distributed mainly in the basal part of the gastric mucosa,
especially in the fundus parts. |
