| Abstrak/Abstract |
Sugarcane (Saccharum officinarum) is one type of sugar-producing plant in Indonesia. Sugar is commonly used as a staple and raw material for the food and beverage industry. This plant is one of the contributors to livelihoods for millions of farmers and as contributors to the national economy. Over the years, there has been an increase in the consumption of sugar as a result of the increasing population in Indonesia. At present, the development of molecular biology techniques is growing so that this causes analysis at the gene level is very important to be done as one of the ways in improving plant breeding programs, especially sugarcane. Research on molecular biology techniques in Indonesia has not been able to provide information on the analysis of molecular identification of the Sus-2 gene encoding high sucrose levels in sugarcane. This information is important because it can increase knowledge about molecular identification of a type of plant and can directly be the application of molecular biology techniques to improve the nature of a plant. This study aims were to determine the differences in phenotypic and molecular characters of sample plants based on the Sus-2 gene and to determine the specific band profile for detection of high sucrose sugarcane plants. The method used was PCR method with AI-specific primers, qualitative test of PCR analysis by electrophoresis technique, quantitative test with spectrophotometry. Based on the research that has been done it can be seen that cultivars PS 80.910, PS 80.1649, PSCO 902, PSBM 901, PSJT 941, BL, Kentung, KK, BZ 132, TLH 2, PSDK 923, and VMC 76-16, are sugarcane cultivars that have not high sucrose. This can be proven from the profile of the sugarcane band not produced amplicons in 500 bp when using a primary AI. |
