| Abstrak/Abstract |
Sari MA, Retnaningrum E. 2019. Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil
mining site in Wonocolo, Bojonegoro, Indonesia. Biodiversitas 20: 3422-3429. High oil mining activities cause environmental pollution
at the oil mining site. Petroleum spills and waste to the river flow cause blackish discoloration along the riverbank and also some types
of plants die. The utilization of rhizospheric bacteria (rhizobacteria) as a bioremediation agent is considered as appropriate for reducing
petroleum contaminants. The purposes of this study were to analyze the ability of hydrocarbon degradation by selected rhizobacteria
isolates, to identify molecularly and analyze the genetic relationship of hydrocarbon-degrading rhizobacteria based on 16S rRNA gene
sequencing. The rhizobacteria of cogongrass (Imperata cylindrica L.) were isolated from oil mining site in Wonocolo, Kedewan,
Bojonegoro, East Java, Indonesia. The 5 isolates of rhizobacteria were then cultured on Bushnell-Haas Mineral Salt (BHMS) medium
with the addition of 0.015% crude oil for analyzing their growth based on their cell densities using the Total Plate Count method. The
optimal rhizobacteria growth obtained (MTMW2 isolate) was selected and further analysed its ability for hydrocarbon degradation
through measuring of Total Petroleum Hydrocarbon (TPH). Its rhizobacteria were then identified using 16S rRNA gene sequencing. The
MTMW2 isolate revealed specific growth rate of 0.14 in BHMS medium with the addition of 0.015% crude oil. After 10 days of
incubation, the isolates could degrade Total Petroleum Hydrocarbon in the medium as much as 96.1%. The results of molecular
identification and phylogenetic tree construction showed that MTMW2 isolate was identified as Pseudomonas aeruginosa.
Keywords: 16S rRNA gene, hydrocarbons biodegradation, phylogenetic tree, Pseudomonas aeruginosa, rhizobacteria |
