High VEGF Level is Produced by Human Umbilical Cord-Mesenchymal Stem Cells (hUC-MSCs) in Amino Acid-Rich Medium and under Hypoxia Condition
Penulis/Author
Veronika Maria Sidharta (1); dr. Elizabeth Henny Herningtyas, M.Si., Ph.D. SpPK(K) (2); Christine Ayu Lagonda (3); Dilafitria Fauza (4); Yuyus Kusnadi (5); dr. Rina Susilowati, Ph.D. (6); Prof. dr. Ginus Partadiredja, M.Sc, Ph.D. (7)
Tanggal/Date
2018
Kata Kunci/Keyword
Abstrak/Abstract
Background: Secretome production by stem cells depends on their culture conditions such as oxygen concentration and the composition of the culture media. In this study, we investigated the secretion of neurotrophic growth factors of human umbilical cord mesenchymal stem cells (hUC-MSCs) in amino acid-rich culture medium and under hypoxic condition.
Methods: hUC-MSCs were cultured in normoxic and various hypoxic (1%, 5%, 10%) conditions in an amino acid-rich culture medium. The end-point parameters (cell proliferation and survival, cell morphology and growth factor secretion) were measured at 3 time-points (48 h, 72 h and 96 h). ELISA-based methods were used for neurotrophic factors detection, including neurotrophic growth factor (NGF), vascular endothelial factor (VEGF), and brain-derived neurotrophic factor (BDNF).
Results: NGF secretion was not detectable at any time points both in normoxia and hypoxia. BDNF secretion under normoxia was induced at 48 h time point and reached the highest level at an average of 181.9 ± 13.01 pg/mL at 96 h, whereas hypoxia exposure to hUC-MSCs only induced the BDNF secretion at low level. This finding in the hypoxia groups than in the normoxia group. VEGF secretion was barely detectable in normoxic condition. However, VEGF secretion reached the highest level at an average of 7707.55 ± 2110.85 pg/mL in 5% hypoxia at 96 h
