| Abstrak/Abstract |
Objectives: High-fat and fructose diet contribute to the development
of hypertriglyceridemia. Sterol regulatory binding protein-1c (SREBP-1c) is involved in the regulation of lipogenic gene expression. A high-fiber diet was reported to suppress SREBP-1c gene expression. The objective is to evaluate the effect of pumpkin and sweet potatoes fibers on SREBP-1c gene expression and triglyceride in dyslipidemic rats. Methods: Twenty-five male Wistar rats were divided into 5 groups: 1) normal (N); 2) high fat and fructose diet (HFFD); 3, 4, and 5) HFFD with substitution of pumpkin and sweet potato containing fiber (g) per day: 20% (2.6 g), 40% (5.2 g), and 60% (7.8 g), respectively. The triglyceride levels were measured before and after
the intervention, whereas SREBP-1c expression in the liver and white adipose tissues were measured at the end of the intervention. HFFD-inducted dyslipidemia was performed 7 weeks and high fiber diet intervention for 6 weeks. Results: Pumpkin and sweet potatoes substitution reduced triglyceride levels in 25%, 47%, and 49% of dyslipidemic rats after 6 weeks of intervention. This intervention also suppresses the expression of the SREBP-1c gene in the liver by dose-dependent but not on white adipose tissue. Conclusion: Pumpkin and sweet potatoes decreased triglyceride of dyslipidemic rats through suppression of SREBP-1c gene expression in liver and white adipose tissues. |
