| Penulis/Author |
AGIL NOVIANTO (1); Prof. Dr. apt. Arief Nurrochmad, M.Si., M.Sc. (2) ; Prof. Dr. Ika Puspita Sari, S.Si., M.Si., Apt. (3); Prof. Dr. Puji Astuti, S.Si., M.Sc., Apt. (4) |
| Abstrak/Abstract |
The present study was carried out to evaluate the hepatoprotective effect of the combination of Curcuma domestica Val and Phyllanthus niruri Linn extract against paracetamol-induced liver injury in Wistar rats. The different groups of animals were administered with vehicle (Na CMC 0.5%), sylimarin (100 mg/kg BW), C. domestica (100 mg/kg BW), P. niruri (200 mg/kg BW), combination of C. domestica and P. niruri (75:50; 50:100; and 25:150 in mg/kg BW) for 7 consecutive days. Thirthy minutes after last treatment all groups were terated with paracetamol (2.5 g/kg BW). The effect of sylimarin, C.domestica, P. niruri, and its combinations on serum transaminase (ALT, AST), alkaline phosphates (ALP), bilirubin, and total protein were measured in paracetamol induced hepatotoxicity in rats. Further, the effects of the extracts or combinations of extracts on liver gluthatione content, lipid peroxidation (LPO), and catalase (CAT) were estimated. Sylimarin, C.domestica, P. niruri, and its combinations produced significant (P<0.05, P<0.01, P<0.001) hepatoprotective effect by decreasing the activity of serum enzymes, bilirubin, and lipid peroxidation and significantly (P<0.05, P<0.01, P<0.001) increased the levels of total protein, CAT, and GSH. The optimum combination dose of C. domestica and P. niruri was 75:50 in mg/kg BW compare to the extracs alone. It was suggested that the combination of C. domestica and P. niruri showed significant hepatoprotective effect in paracetamol-induced liver injury in Wistar rats. |
