Growth Inhibition, Apoptosis Induction, and Antiangiogenesis Activity of Ethanolic Extract of Dendrobium crumenatum Swartz
Penulis/Author
Dr. Ardaning Nuriliani, S.Si., M.Kes. (1); Dr.biol.hom. Nastiti Wijayanti, S.Si., M.Si. (2); Prof. Dr. Yekti Asih Purwestri, S.Si., M.Si. (4)
Tanggal/Date
2013
Kata Kunci/Keyword
Abstrak/Abstract
Genus Dendrobium is widely known as traditional medicine that has potency as antihyperglicemia, antiinflammation, and anticancer. This genus has a lot of antioxidant compounds that potencial as anticancer. Dendrobium crumenatum Swartz. (pigeon orchid), one of tropical orchids is not widely explore yet. This orchid is guessed having anticancer potency. Thus this research was aimed to study the effect of ethanolic extract of Dendrobium crumenatum on growth inhibition and apoptosis induction on HeLa cells, as well as antiangiogenesis activity on chorio allantoic membrane (CAM) of chicken. Cell growth inhibition was done using MTT assay. Apoptotic assay was done using double staining method (ethidium bromide-acridine orange). Antiangiogenesis test was done on chorio allantoic membrane of chicken with bFGF as inductor of angiogenesis. The results showed that the IC50 of three part of D. crumenatum i.e leaves, pseudobulb, and whole plant on HeLa cell growth inhibition were 153.828; 166.284; and 192.298 ?g/mL respectively. The apoptosis induction and antiangiogenesis activity were done using leaves ethanolic extract of D. crumenatum because of the smallest IC50 value. The percentage of apoptotic cells on the treatment showed pattern based on concentration. Nevertheless at the highest concentration of leaves ethanolic extract of D.crumenatum the mean of apoptotic cells just 27.69 ± 10.22%. In this research angiogenesis was induced by bFGF as a positive control. Antiangiogenesis activity of leave ethanolic extract of D. crumenatum showed that inhibition value was 64.77% at the concentration of 76.914 ?g/mL. Based on this research it could be concluded that ethanolic extract of D. crumenatum has potency as anticancer agent.
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Lain-Lain
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Internasional
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GROWTH INHIBITION, APOPTOSIS INDUCTION, AND ANTIANGIOGENESIS ACTIVITY OF ETHANOLIC EXTRACT OF Dendrobium crumenatum Swartz.pdf