| Abstrak/Abstract |
Nanoparticles have great potential in medicinal aspects. Among metal nanoparticles,
silver nanoparticles (AgNPs) are very popular because of their ability to act in many
treatments such as antibacterial, antifungal, antioxidant, anticancer, antidiabetic, and
anti-inflammatory. In this study, we synthesized AgNPs by using a safe and non-toxic
method (green method) using petai seed extract. Extract can be useful as a reductor
and stabilizer agent in the synthesis of AgNPs. The AgNPs were characterized by UVvis spectroscopy, zetasizer, SEM-EDX, TEM, and FTIR. The antibacterial effects of
The AgNPs were studied by disk diffusion and microdilution methods against
Staphylococcus aureus and Escherichia coli. The UV-vis spectrum showed a peak at
424 nm, an average particle size of 116.5±1.07 nm with PDI of 0.193±0.01, and a zeta
potential of –13.5±0.2 mV. The SEM-EDX and TEM confirmed that AgNPs have a
spherical shape. The FTIR spectra showed a shift in the AgNPs wavelength which
confirmed the reduction process. Moreover, the disk diffusion test showed that AgNPs
and silver nitrate at a concentration of 5000 µg/mL create inhibition zone of 13.4±3.8
and 11.5±0.5 for S.aureus; 11.9±1.3 and 13.1±1.4 mm for E.coli, respectively.
Microdilution test showed that kanamycin, AgNPs, and silver were able to inhibit the
growth of S.aureus with MIC respectively of 11.5, 115.6, and 2797.3 µg/mL; and E.coli
with MIC respectively of 4.0, 1264.3, 1771.0 µg/mL. These results suggest that AgNPs
can be synthesized by using petai seed and have the ability as an alternative
antibacterial agent. |
