Goat-Soy Milk Kefir Increase Nitric Oxide Bioavailability by Increasing Endothelial Nitric Oxide Synthase (eNOS) Gene Expression in Diabetic rats
Penulis/Author
Alfi Muntafiah (1); Prof. Dr. Ir. Nurliyani, M.S., IPM (2); Prof. Dr. Dra. Sunarti, M.Kes. (3)
Tanggal/Date
20 2018
Kata Kunci/Keyword
Abstrak/Abstract
Objective: Hyperglycemia-stimulated oxidative stress in diabetes mellitus impairs gene expression
and activity of endothelial nitric oxide synthase (eNOS). Goat-soy milk kefir has potential to reduce hyperglycemia.
This study is to investigate the benefit of kefir from goat and soy milk on eNOS gene expression and
nitric oxide (NO) levels. Methods: Twenty five male Wistar rats were grouped as: 1) normal rats; 2) diabetic
rats; 3) diabetic rats + goat milk kefir; 4) diabetic rats + combination of goat-soy milk kefir; 5) diabetic rats +
soy milk kefir. After kefir intervention for 4 weeks, eNOS gene expression was determined using q-PCR and
NO levels was analyzed by ELISA methods. Results: There were significantly differences of the NO levels
among group after intervention of kefir for 4 weeks, and the highest NO level was found the diabetic rats
with combination of goat-soy milk kefir (p<0.05). However, the eNOS gene expression was found highest in
the diabetic rats with soy milk kefir. Conclussion: The combination of goat-soy milk kefir had good effects on
expression of eNOS, NO levels and plasma glucose levels in diabetic rats.
