Karya
Judul/Title Genomic analysis and identification of a novel superantigen, SargEY, in Staphylococcus argenteus isolated from atopic dermatitis lesions
Penulis/Author drh. Fatkhanuddin Aziz, M.Biotech., Ph.D (1); Junzo Hisatsune (2); Hisaya K. Ono (3); Junko Kajimura (4); Liansheng Yu (5); Kanako Masuda (6); Hiroki Kitagawa (7); Yusuke Sato'o (8); Koji Yahara (9); Mika Yamaoka (10); Akio Nakane (11); Hiroshi Kawasaki (12); Shoko Obata (13); Ayano Fukushima-Nomura (14); Yoshihiro Ito (15); Meiji Soe Aung (16); Masayuki Amagai (17); Prof. Dr. drh. Siti Isrina Oktavia Salasia (18); Hiroki Ohge (19); Yoichiro Kusunoki (20); Motoyuki Sugai (21)
Tanggal/Date 11 2024
Kata Kunci/Keyword
Abstrak/Abstract During surveillance of Staphylococcus aureus in lesions from patients with atopic dermatitis (AD), we isolated Staphylococcus argenteus, a species registered in 2011 as a new member of the genus Staphylococcus and previously considered a lineage of S. aureus. Genome sequence comparisons between S. argenteus isolates and represen- tative S. aureus clinical isolates from various origins revealed that the S. argenteus genome from AD patients closely resembles that of S. aureus causing skin infections. We previously reported that 17%–22% of S. aureus isolated from skin infections produce staphylococcal enterotoxin Y (SEY), which predominantly induces T-cell proliferation via the T-cell receptor (TCR) Vα pathway. Complete genome sequencing of S. argenteus isolates revealed a gene encoding a protein similar to superantigen SEY, designated as SargEY, on its chromosome. Population structure analysis of S. argenteus revealed that these isolates are ST2250 lineage, which was the only lineage positive for the SEY-like gene among S. argenteus. Recombinant SargEY demonstrated immunological cross-reactivity with anti-SEY serum. SargEY could induce proliferation of human CD4+ and CD8+ T cells, as well as production of TNF-α and IFN-γ. SargEY showed emetic activity in a marmoset monkey model. SargEY and SET (a phylogenetically close but uncharacterized SE) revealed their dependency on TCR Vα in inducing human T-cell proliferation. Additionally, TCR sequencing revealed other previously undescribed Vα repertoires induced by SEH. SargEY and SEY may play roles in exacerbating the respective toxin-producing strains in AD.
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
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