Genetic Variability and Phylogenetic Relationships of Begonia multangula Based on atpB-rbcL Non-Coding Spacer of cpDNA Sequences
Penulis/Author
TRI WARSENO (1); Muhammad Efendi (2); Abdul Razaq Chasani, S.Si., M.Si., Ph.D. (3); Prof. Dr. Budi S. Daryono, M.Agr.Sc. (4)
Tanggal/Date
2022
Kata Kunci/Keyword
Abstrak/Abstract
Begonia multangula (Begoniaceae) belongs to Section Platycentrum-Sphenanthera Group which has a wide distribution from Sumatra to the Lesser Sunda Islands and Sulawesi. Information on B. multangula genetic variability and intraspecies relationships based on molecular characters is critical for developing appropriate strategies in conservation biology, breeding activities, and many other applied fields. The genetic
variability and phylogenetic relationships of B. multangula in Indonesia were investigated using sequence data from the atpB-rbcL intergenic spacer (IGS) cpDNA regions. The atpB-rbcL IGS fragment was amplified using atpB-1 as the forward primer and rbcL-1 as the reversed primer. Genetic variations were found in the length of the sequence and nucleotide divergences in the atpB-rbcL IGS region. The genetic distance between 822 fixed sites ranged from 0 to 0.61 %. Eighteen of the 822 sites (99.27 %) analyzed were invariable, six sites (0.73%) were variable consisting of four singleton variable sites and two parsimony informative sites, and twelve sites were gaps. The phylogenetic relationships generated by B. multangula based on the atpB-rbcL IGS sequence analysis indicated the genetic variation and divided it into two clades. However, the clustering pattern of B. multangula specimens resulting from molecular analysis based on atpB-rbcL IGS sequences did not show the geographic clustering grouping pattern.
Rumpun Ilmu
Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Publikasi_Biodiversitas 2022_Tri Warseno.pdf
[PAK] Full Dokumen
2
Surat Keterangan-Genetic variability and phylogenetic relationships of Begonia.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
3
Cek Similaritas-Genetic variability and phylogenetic relationships of Begonia multangula based on atpB-rbcL non-coding spacer of cpDNA sequences.pdf
[PAK] Cek Similarity
4
BUKTI KORESPONDENSI_Phylogenetic Relationships of Begonia multangula.pdf
