Karya
Judul/Title Genetic homogeneity of Anopheles maculatus in Indonesia and origin of a novel species present in Central Java
Penulis/Author Triwibowo Ambar Garjito (1) ; Umi Widiastuti (2); Mujiyono (3); Mega Tyas Prihatin (4); Widiarti (5); Riyani Setyaningsih (6); Siti Alfah (7); Dr. Barandi Sapta Widartono, S.Si., M.Si., M.Sc. (8); Din Syafruddin (9); Prof. dr. Tri Baskoro Tunggul Satoto, M.Sc., Ph.D. (10); Laurent Gavotte (11); Michael J. Bangs (12); Sylvie Manguin (13); Roger Joseph Frutos (14)
Tanggal/Date 2019
Kata Kunci/Keyword
Abstrak/Abstract Abstract Background: Anopheles maculatus (s.s.) is an important vector of malaria in Indonesia. Previously it was considered the only member of the Maculatus Group present in Indonesia. A novel species was recently identifed in the Kulon Progo District in Central Java. Until recently, few investigations have been conducted looking at An. maculatus genetic diversity in Indonesia, including allopatric island populations. Methods: Indonesian An. maculatus (s.l.) samples were collected in several locations in Java, Lesser Sunda Island group, Sumatra and in Kulon Progo (Yogyakarta, central Java) where a novel species has been identifed. Samples from a 30-year-old colony of the Kulon Progo population were also included in the analysis. Maximum-likelihood analysis established the phylogenies of the ITS2 (nuclear) and cox1 (mitochondrial) markers. Putative times of separation were based on cox1 genetic distances. Results: Two species of the Maculatus Group are present in Indonesia. The novel sibling species is more closely related to Anopheles dispar than to An. maculatus (s.s.). Anopheles maculatus (s.s.) samples are homogeneous based on the ITS2 sequences. Indonesian samples and An. dispar belong to the same cox1 maternal lineage and difer from all other known members of the Maculatus Group. Divergence time between the diferent populations found in Java was estimated using an established cox1 mutation rate. Conclusions: A novel species within the Maculatus Group, most closely related to An. dispar, is confrmed present in the Kulon Progo area of Central Java. The divergence of this species from An. maculatus (s.s.) is explained by the stable refugia in the Kulon Progo area during the quaternary period of intense volcanic activity throughout most of Java. This novel species awaits detailed morphological description before applying a formal species name. For the interim, it is proposed that the Kulon Progo population be designated An. maculatus var. menoreh to distinguish it from An. maculatus (s.s.).
Rumpun Ilmu Bidang Kedokteran Spesialis Lain Yang Tercantum
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
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