Genetic Diversity of Pyriculariaoryzae Cause Rice Blast Disease by Using Repetitive Element-Based Polymerase Chain Reaction
Penulis/Author
DANAR WICAKSONO (1); Dr. Ir. Arif Wibowo, M.Agr.Sc. (2); Prof. Ani Widiastuti, S.P., M.P., Ph.D. (3)
Tanggal/Date
2017
Kata Kunci/Keyword
Abstrak/Abstract
Pyricularia oryzae is a pathogen that causes rice blast disease. One of the challenges in controlling rice blast disease is the high diversity of P. oryzae. This study was conducted to analyze the genetic diversity of P. oryzae. Eleven samples were collected from the Special Region of Yogyakarta, nine samples from Central Java Province, and one sample from West Java Province, Indonesia. DNA samples of P. oryzae isolates were fingerprinted by using repetitive element–based polymerase chain reaction (rep-PCR) with two primers, Pot2–1 and Pot2–2. The results showed 25 amplified bands ranging in size from ±600 bp to ±3400 bp. A dendrogram showed that samples taken from nearby places did not always have high similarity. The dendrogram showed that all isolates were grouped in six clusters at a 70% similarity level. These results showed that the genetic diversity of P. oryzae is high and spread randomly.
