Genetic Diversity of Mudskipper Species (Periophthalmus spp.) from the Southern Coast of Java, Indonesia, Based on the Mitochondrial 16S Gene
Penulis/Author
Prof. Dra. Tuty Arisuryanti, M.Sc., Ph.D. (1); TASYA NURALIYAH (2); Vindi Dwijayanti (3); KATON WASKITO AJI (4); Dr. Dwi Sendi Priyono, S.Si., M.Si. (5); Prof. Dr. Budi S. Daryono, M.Agr.Sc. (6)
Tanggal/Date
2025
Kata Kunci/Keyword
Abstrak/Abstract
The genus Periophthalmus (Bloch & Schneider, 1801) comprises a diverse group of
mudskippers within the family Oxudercidae and subfamily Oxudercinae. It includes
20 verified species globally, with 11 of these species originating from Indonesia.
Among them, P. kalolo and P. argentilineatus are prevalent across seven major
islands. However, P. novemradiatus is less documented, with limited distribution
records. The morphological similarities among Periophthalmus spp. complicate
recognition, often leading to misidentification. To address this challenge, this study
investigated the phylogenetic relationships and genetic diversity among these three
species, utilizing the 16S rRNA gene as a marker. PCR amplification of the DNA
from samples collected from seven regions along Java's southern coast employed
primers 16Sar and 16Sbr. Phylogenetic analysis revealed three monophyletic clades
corresponding to each species, with genetic divergences ranging from 3.66 to 5.40%.
P. argentilineatus displayed a relatively high intraspecific divergence of 0.21-2.26%,
suggesting the potential identification of cryptic species within this lineage, even with
a conserved gene like 16S rRNA. We identified 47 variable sites within the 552-bp
16S rRNA sequence, including four singleton sites and 43 parsimony-informative
sites. We discovered a unique marker at position 172 that could serve as a genetic
identifier for distinguishing these species. These findings suggest that the 16S rRNA
gene has potential as an alternative marker for species identification while also
revealing genetic diversity, thereby complementing or even serving as an alternative
to the commonly used COI gene in Periophthalmus spp.
