GENETIC DIVERSITY OF GREEN CHIRETA (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) FROM INDONESIA BASED ON ISSR AND RAPD MARKERS
Penulis/Author
MUHAMMAD FAUZI ARIF (1); Dyah Subositi (2); ANISKA NOVITA SARI (3); Ganies Riza Aristya, S.Si., M.Sc., Ph.D. (4); Indra Lesmana, S.Si., M.Sc. (5); Prof. Rina Sri Kasiamdari, S.Si., Ph.D. (6)
Tanggal/Date
30 2020
Kata Kunci/Keyword
Abstrak/Abstract
Green chireta or Andrographis paniculata (Burm.f.) Wall. Ex Nees. is a medicinal plant widely used by traditional communities in Indonesia. The purposes of this study were to study and analyze the genetic diversity of A. paniculata in five mainlands Indonesia using ISSR and RAPD markers. A total of 50 accessions of A. paniculata were amplified using six ISSR and six RAPD primers, generating 61 loci and 43 loci respectively. Scoring data were used to determine the percentage of polymorphism, Polymorphic Information Content, Shannon's Information Index, unbiased expected heterozygosity. The genetic variation between the locations was measured with Principal Coordinate Analysis (PCoA) and pairwise Nei’s unbiased genetic distance. The data from this study revealed that the variation and diversity from accession within the same island were low. The genetic variation was higher between accessions from different islands. Analysis of molecular variance (AMOVA) was used to calculate the distribution of the variation between and within the A. paniculata population. The result showed the variation derived from the same location was 34% and from a different location was 66%. Genetic diversity provided information to help the further development and cultivation of A. paniculata in Indonesia.
Keywords: Andrographis paniculata, genetic diversity, ISSR, medicinal plant, RAPD
Rumpun Ilmu
Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Full_2020_Genetic Diversity of Green Chireta (Andrographis paniculata (Burm_f_) Wall_ Ex_ Nees_) from Indonesia Based on ISSR and RAPD Markers - PAK Rina SK.pdf
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