| Abstrak/Abstract |
Indonesia occupies the top 10 tea producers in the world with a plantation area of 104,420 hectares in 2018 with a production of 139,285 thousand tons in the same year. The trend is increasing, but climate change affects fluctuating tea production due to the development of blister blight. This long-standing disease is caused by Exobasidium vexans. Knowledge of genetic diversity studied in this study is the basis for selecting superior parents and controlling appropriate clusters.
Sampling was conducted at one of the tea plantations in Indonesia, Pagilaran in Central Java with sampling based on altitude, Andongsili (>1,000 masl), Kayulandak (1,000 masl), and Pagilaran (<1,000 masl) with clones TRI 2024, TRI 2025, Gambung 3, Gambung 7, Gambung 9, and Pagilaran 15. This study used the PCR method to obtain E. vexans sequences using ITS 1F and ITS 4 primers. Genetic diversity was tested using PCR-RAPD methods. Five primers used in PCR-RAPD were OPA-01, OPA-02, OPA-03, OPA-05, and OPB-17. Morphological symptoms of tea blister blight caused by Exobasidium vexans between clones at each altitude did not show any difference. PCR-RAPD method can show the diversity of E. vexans samples between clones, namely at a coefficient of 0,63 three clusters were formed, Cluster I consisted of TRI 2024 Andongsili and PGL 15 Pagilaran; Cluster II consisted of TRI 2025 Andongsili and Gambung 3 Andongsili; Cluster III consisted of Gambung 7 Andongsili, Gambung 7 Kayulandak, and Gambung 9 Andongsili. |
