Abstrak/Abstract |
Jackfruit (Artocarpus heterophyllus Lam.) is one of the most widely cultivated fruit
in Indonesia. The Faculty of Forestry, Gadjah Mada Universtity, Indonesia has 11
provenances where jackfruitis widely grown in Indonesia. The aim of this study was
to assessed variation injackfruit based on molecular marker RAPD (Random
Amplified Polymorphic DNA). Seven RAPD primers namely A-13, A-27, B-4, H-15,
L-1, OPB-1, and OPD-19 generated 70 DNA bands, of which 40 were polymorphic.
In this study H-15 primers produced more polymorphic fragments than the other
six primers and had the highest percentage of polymorphic fragments, namely
91.7%. Similarity matrix was calculated using coefficient of Jaccard. Unweighted
Pair Group Method Using Arithmetic Mean (UPGMA) cluster analysis was
performedto develop a dendogram. This data analysis was performed by NTSYS
software ver. 2.1. In the present studies, jackfruit tress accessions from the eleven
provenances were divided into two clusters with similarity coefficient ranging from
63.33 to 85.71%, provenance of East Java and West Java were the most closely
similar (85.71%) based on molecular characters.
Key words: Jackfruit, molecular characters, genetic diversity, RAPD |