Genetic diversity and taxonomic relationships of teak (Tectona grandis L.f) provenances in Indonesia based on Random Amplified Polymorphic DNA analysis
Penulis/Author
Abdul Razaq Chasani, S.Si., M.Si., Ph.D. (1); Prof. Dr. Ir. Taryono, M.Sc. (2)
Tanggal/Date
2009
Kata Kunci/Keyword
Abstrak/Abstract
Teak (Tectona grandis L.f.) is one of the valuable and economic plant in Indonesia due to their excelent wood structure and figure. Teak is recognized as a luxury timber around the world. The research and development program on Teak should be equally balanced with deeper understanding of the biological characters including their variations and taxonomic relatonships. Accordingly, this research was conducted to study genetic variations and taxonomic relationships of Teak in Indonesia in order to support the conservation and plant breeding program. In this study, RAPD (Random Amplified Polymorphic DNA) technique, which facilitates detection of variability at DNA level, was used to know the genetic diversity and taxonomic relationships of Teak provenances in Indonesia. A number of Teak provenances from Java and Sulawesi were observed for their DNA fingerprinting using Popgene 3.2. and NTSys 2.02i. The provenance relationships was shown as a dendrogram based on cluster analysis. The information on Teak origin and their possible genetic drift in Indonesia was done by literature study. Result showed that the genetic variation of Teak within population was 62.22%, which is higher than between population (37.78%). Provenance relationships indicated that the Indonesian Teaks were clustered into two groups, Java and Sulawesi, with genetic distance of 10.51%. The clusters obtained, however, did not clearly differentiate provenance based on their origin. It was assumed that Indonesian Teak not only consists of Tectona grandis but there is another species of Teak i.e. Tectona hamiltoniana
