| Abstrak/Abstract |
A scaffold that mimics physicochemical structure of nerve and supplies calcium ions in
axonal environment is an attractive alternative for nerve regeneration, especially when
applied in critical nerve defect. Various scaffold material, design, including their
combination with several growth- induced substances and cells application have been
being investigated and used in the area of nerve tissue engineering. However, the
development remains challenges today because they are still far from ideal concerning
their stability, reproducibility, including complicated handling related to the poor
mechanical strength. In view of the current basis, in this study the introduction of
carbonated hydroxyapatite (CHA) as promising candidate to increase mechanical
properties of nerve scaffold is reported. The incorporation of CHA was not only
expected to provide better mechanical properties of the scaffold. As the most stable
phases of calcium phosphate compound under physiological condition, CHA was also
prepared to provide better axonal environment with calcium released in a controllable
manner, therefore, to promote fasten nerve regeneration. This study shows that CHA
introduced gelatin membrane has ideal microstructure to prevent fibrous tissue ingrowth
into the injury site, while retaining its capability to survive nerve tissue, by allowing
adequate glucose and specific proteins diffusion. The provided Ca2+ release to the
environment promoted neuronal growth, without suppressing acetylcholine esterase
release activity. Neurite elongation was dramatically higher in the gelatin membrane
incorporated with CHA in the system. Introduction of CHA into gelatin membrane
represents a new generation medical device for nerve reconstruction, with CHA was
considered as a promising factor. |
