| Abstrak/Abstract |
Research on taxonomy of Lasiodiplodia spp. are well developed recently in all over the world. Some Botryosphaeria anamorph had been characterized based on DNA structure as new species of Lasiodiplodia spp. There were even three species originally from tropical area, Australia and Venezuela, had been successfully characterized and named as L. venezuelensis, L. crassispora and L. rubropurpurea.
The fruit rot symptom caused by Lasiodiplodia spp. are frequently similar to fruit rot caused by other pathogen, moreover the infection often occurs together with other fruit rot causal agents. This research was undertaken to observe the symptom of fruit rot caused by Lasiodiplodia spp. and to collect their pure culture isolated from a single spore. Methods in this research were sample collection of various symptom of fruit rot, single-spore isolation for pure culture collection, morphology identification based on literature, and inoculation test. Result showed that Lasiodiplodia spp. were found in the largest amount of samples which attacked seven from 17 observed fruits. From all fruit rot observed, the pure culture of Lasiodiplodia spp. are isolated from mangosteen, avocado, mango, orange, and cacao fruit. Inoculation test resulted the same symptom as previous symptom which were either fruit mumification or bottom rot. |
