| Abstrak/Abstract |
Excessive free radicals in the body can trigger the growth of cancer cells,
coronary heart disease, brain oxidative damage and premature aging. This
led the increase of research on the antioxidant subtans, especially natural
antioxidants from plants. Kecombrang flower (Nicolaia speciosa (Bl.)
Horan) and talok fruit (Muntingia calabura L.) were reported active as an
antioxidant and contains active compounds with different levels of polarity.
Therefore, this study aims to test the free radicals scavenging activity from
fractions of kecombrang flower and of talok fruit extracs. It follows to
characterize the type of active compound. The fractionated extracts were
tested by 0.4 mM DPPH solution and measured by UV-Vis
spectrophotometer at 517 nm wavelength. The absorbance of samples and
controls were calculated to obtain the percent of radical DPPH scavenging
and antioxidant activity was evaluated by IC50 values. Active compounds
were identified using thin layer chromatography with FeCl3 , AlCl3, 2,4-
DNPH and anisaldehid-H2SO4 spray reagents. Fractions of kecombrang
flower (BK) and talok fruit (BT) extract showed antioxidant power level
between moderate to very active. Ethyl acetate fraction had the highest free
radical scavenging activity with IC50 29.81 ?g/ml (BK) and 14.48 ?g/ml
(BT). For kecombrang flower, the water fraction had this activity with IC50
39.27 ?g/ml, followed the ethanol extract with IC50 44.08 ?g/ml, then the
hexane fraction with IC50 135.36 ug/ml. Chromatogram of ethyl acetate
fraction showed that spots on the hRf 12 and 56 were thought to contain
phenolic compounds with a carbonyl group, while the hRf 37 was
suspected flavone compounds with 3-OH group and the hRf 50 was alleged
the ortho-dihydroxy flavone or ortho-hydroxy and free-carbonyl. For talok
fruit, chromatogram of ethyl acetate fraction showed that spots on the hRf
19, 31 and 44 were suspected flavone compounds with ortho-hydroxy-
carbonyl group and or ortho-hydroxy. |
