| Abstrak/Abstract |
Using cur cumin as anticancer agent is restricted vy its low solubility. The obstacle can be solved by the development of cur cumin nano particle. Nanoparticle technology has been started to be developed as an alternative solution to improve drug delivery profile, especially for the less bioa-available chemical. This study was aimed to develop nanocurcumin with formulation with low viscosity chitosan as the matrix and to study its ability to be taken into cell in vitro. Method used in the formulation of nanocurcumin in this study is ionic gelation followed by freeze drying. Entrapment efficiency then assayed, and its stability was tested by incubating the formula into artficial intestinal fluid (AIF). Further, its toxicity was evaluated, also its cellular uptake ability into T47D cell line. Ot was found that entrapment efficiency in acetate buffer pH 4 is higher than in pH 5. The formula has good stability in IF. For the cellular uptake study by fluorescence microscope, it was found that the complex has ability to penetrate the cellular membrane. Cytotoxocity study showed that nanocurcumin non toxic to normal cell line. Caharacterization of nano particles shows that average size is 269.8 nm, its zeta potential is +18.63, with spherical morphology. From the results, it is concluded that formulation of nanocurcumin using low viscosity chitosanpolymer has a great potency as an alternative for anticancer therapy. |
