| Abstrak/Abstract |
Applications insulin orally as an alternative to insulin injections, encounter obstaclessuch as degradation by protease enzymes and insulin poor permeability inthe gastrointestinal tract. One solution offered is insulin formulation into the nanoparticles form. In this study, nanoparticle formulation of insulin used polymers combination of low molecular weight chitosan and pectin. The aim of this study was to obtain the optimum formula of insulin nanoparticles that can be used as an alternative therapy for patient with DM. Preparation of insulin nanoparticles was carried out by ionic gelation method utilizingpolyelectrolyteinteractionbetween(-NH3+)ofchitosanand(-COO-)ofpectintoformnanoparticlesthatare compact and stable charge. Formula optimizationwas performed using Factorial Design 22 with Design Expert®7.1.5 software.ConcentrationandpHofthepectinwereusedasfactors,whiletheentrapmentefficiency,particlesizeand polydispersityindexwereusedasresponses.Theoptimumformulawasfurtherevaluatedlikezetapotential,particle morphology, profile spectra of FT-IR and in vitro release study.The obtained optimum formula consist of chitosan of 0.05% and 0.4% pectin (pH 5.0) with the mean entrapment efficiency of 63,59% ± 2,17, particle size of 228,3 nm ± 26,3, polydispersityindexof0,354±0,042,zetapotential49,40mV±11,59,particleroundanddark,ionicgelationwas confirmed by FT-IR and the release profile following the kinetics Korsmeyer-Peppas models with Fickian diffusion release mechanism on media HCl buffer pH 1,2 (n = 0,363) and (n = 0,318) on PBS buffer pH 6,8 were analyzed by DDSolver program. |
