Karya
Judul/Title Field and Laboratory Detection of Clove Sumatra Disease Caused by Ralstonia syzygii subsp. syzygii in Indonesia
Penulis/Author Prof. Dr. Tri Joko, S.P., M.Sc. (1) ; ARZAQ PRABANTORO Y (2); RESTU INDRAWATI (3); Alan Soffan, S.P., M.Sc., Ph.D. (4); Prof. Dr. Ir. Siti Subandiyah, M.Agr.Sc. (5)
Tanggal/Date 30 2023
Kata Kunci/Keyword
Abstrak/Abstract The significant decrease in clove production in Indonesia is mainly due to the Sumatra disease caused by bacterial Ralstonia syzygii subsp. syzygii. It is necessary to manage the disease on a broad scale based on disease detection both in the field and in the laboratory. This study aimed to determine the technique for detection the distribution pattern of Sumatra disease using geographic information systems (GIS) and validate the presence of R. syzygii subsp. syzygii in clove plant tissues by molecular analyses based on the endoglucanase (egl) gene. This research was conducted by acquiring aerial photos using unmanned aerial vehicles (UAV) processed using photogrammetric techniques so that geographic information system outputs could be produced in the form of a thematic map of the distribution pattern of clove Sumatra disease. The plant samples were collected for molecular analysis of the pathogens causing clove Sumatra disease in the laboratory. DNA extraction was performed and then amplified by polymerase chain reaction (PCR) using UGMRss-specific primers followed by Sanger sequencing. The aerial photo images showed that the distribution of clove Sumatra disease has a random pattern, which tends to spread between healthy and diseased plants. Furthermore, the PCR and phylogenetic analyses revealed that all symptomatic clove plant samples (leaves, twigs, and roots) were positively infected by R. syzygii subsp. syzygii.
Rumpun Ilmu Ilmu Hama dan Penyakit Tanaman
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1Field and Laboratory Detection of Clove Sumatra Disease Caused by Ralstonia syzygii subsp_ syzygii in Java, Indonesia.pdf[PAK] Cek Similarity
2Tri Joko-Pertanika JTAS.pdf[PAK] Full Dokumen
3Bukti Korespondensi - PJTAS.pdf[PAK] Bukti Korespondensi Penulis