Karya
Judul/Title Expression of Senescence Markers Increased in the Frontal Lobes of Obese Rats
Penulis/Author Prof. Dr. dr. Dwi Cahyani Ratna Sari, M.Kes.PA(K) (1); dr. Mawaddah Ar Rochmah, Ph.D., Sp.N. (2); Wiwit Ananda Wahyu Setyaningsih, S.Keb., M.Sc. (3); JOSEPHINE DEBORA (4); Dr. Dhite Bayu Nugroho, S.Ked. MSc. PhD. (5); dr. Nur Arfian, Ph.D. (6)
Tanggal/Date 2020
Kata Kunci/Keyword
Abstrak/Abstract Introduction: Obesity has been demonstrated to induce oxidative stress and inflammation processes that lead to senescence. Obesity-induced cellular senescence in the brain is still widely investigated. This study aimed to inves-tigate the expression of senescence markers in the frontal lobes of obese rats. Methods: Three groups of rats: control, Obese-2 (Ob-2), and Obese-4 (Ob-4) were observed. Control rats were fed with a standard diet for one month. In contrast, Ob-2 and Ob-4 rats were fed with a high-fat diet daily for two and four months, respectively. After being sacrificed, the rats’ brains were dissected out then the frontal lobes were used for RNA extraction. Reverse tran-scriptase PCR of p-16, p-21, and beta-actin was performed to investigate the relative expression of the senescence markers. Results: Ob-2 and Ob-4 groups had significantly increased body weight after being fed with a high-fat diet for two and four months, respectively. The mRNA expressions of p-16 and p-21 in the frontal lobes of three groups showed similar patterns. The ob-4 group had the highest mRNA expressions of both p-16 and p-21. In comparison to control and Ob-2 groups, the mRNA expressions of p-16 and p-21 were markedly increased. Furthermore, the mRNA expressions of p-16 and p-21 between control and Ob-2 groups were comparable. Conclusion: Increased senescence markers in the rats’ frontal lobes were observed as the chronic effect of obesity.
Rumpun Ilmu Ilmu Kedokteran Dasar & Biomedis
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1FULL DOCUMENT Expression of Senescence Markers Increased in the Frontal Lobes of Obese Rats.pdf[PAK] Full Dokumen
2CEK SIMILARITY Expression of Senescence Markers Increased in the Frontal Lobes of Obese Rats.pdf[PAK] Cek Similarity
3Bukti korespondensi - Expression of Senescence Markers Increased in the Frontal Lobes of Obese Rats.pdf[PAK] Bukti Korespondensi Penulis
4Bukti korespondensi_Expression of Senescence Markers Increased in the Frontal Lobes of Obese Rats.pdf[PAK] Bukti Korespondensi Penulis
5Expression of senescence markers increased in the frontal lobes of obese rats.pdf[PAK] Full Dokumen