| Abstrak/Abstract |
Black rice is an alternative staple food better than white rice. It has lower carbohydrates, but higher anthocyanin compared to white rice. Nowadays, black rice consumption has increased, production needs to be increased to accommodate the demand. However, to our knowledge, there is a lack of information about black rice resistance against biotic stresses, especially Cempo Ireng as one of its cultivars. This information needed for optimal Cempo Ireng cultivation. In this research we applied salicylic acid (SA) treatment to improve black rice resistance to bacterial leaf blight (BLB) caused by Xanthomonas oryzae (Xoo). We determined chlorophyll level as it exhibits the ability of plants in producing yield. We also analyzed rice resistance gene OsNPR1 expression level from three cultivars: Java14 (resistant control), Cempo Ireng, and IR64 (susceptible control), against Xoo. This gene is supposed to suppress chlorophyll production and the photosynthesis process. We sprayed all the plants with SA before inoculated with Xoo. IR64 24h had the lowest chlorophyll level (0.576 ± 0.066 mg/g), meanwhile Java14 72h had the highest level (2.358 ± 1.301 mg/g). OsNPR1 expression did not show any significant change in Java14 and Cempo Ireng after being inoculated. However, IR64 showed increasing OsNPR1 in 72h and did not change in 96h after inoculation. It showed that OsNPR1 played an important role in IR64 resistance against Xoo, but not in Cempo Ireng and Java14. |
